To epitope tag the novel GRAs, ~1,500?bp of the 3 genomic region of each gene was amplified using the primers listed in Table?S6?in the supplemental material (primers p7-p28, p33-p34, and p43-44) from Pru genomic DNA
To epitope tag the novel GRAs, ~1,500?bp of the 3 genomic region of each gene was amplified using the primers listed in Table?S6?in the supplemental material (primers p7-p28, p33-p34, and p43-44) from Pru genomic DNA. at ~97?kDa, which is the predicted size of GRA39 after removal of the 35-amino-acid N-terminal signal sequence. Download Figure?S3, TIF file, TAK-778 2.9 MB mbo004162909sf3.tif (2.9M) GUID:?7C5B8B49-6572-465A-A9A3-EE3981865DDC Figure?S4 : GRA39 mouse infection weights and survival. Graphs show mouse weights (grams) for C57BL/6 mice injected intraperitoneally with the designated doses of GRA39-HA, is an obligate intracellular parasite that invades host cells and replicates within a unique parasitophorous vacuole. To maintain this intracellular niche, the parasite secretes an array of dense granule proteins (GRAs) into the nascent parasitophorous vacuole. These GRAs are believed to play key roles in…