Biol
Biol. two mutually exclusive HIC1 binding sites (HiRE) around the promoter. transcriptional activation induced by short-term serum starvation coincides with loss of occupancy of the distal sites by HIC1/MTA1 and HIC1/CtBP. Upon longer starvation, both complexes are found but on a newly identified proximal HiRE that is evolutionarily conserved and specifically enriched with repressive histone marks. Our results decipher a mechanistic link between two competitive posttranslational modifications of HIC1 and corepressor recruitment to specific genes, leading to growth control. (is usually a direct target gene of P53 (5, 24, 65). HIC1 directly represses the transcription of transcription in response to DNA damage, but SIRT1 binds and deacetylates E2F1 that inhibits E2F1-mediated gene activation (30, 66). In addition, E2F1 directly activates (27) and HIC1 directly represses the promoter in quiescent but…