**** mutant, 2594delC germline mutation in exon 11 and deletion of the wild type allele; RRID: CVCL_B079), UWB1
**** mutant, 2594delC germline mutation in exon 11 and deletion of the wild type allele; RRID: CVCL_B079), UWB1.289 WT (RRID: CVCL_B078) were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA). of OvCa. mutated HGSOC lines: UWB1.289 WT, UWB1.289 MUT, PEO1, OVCAR4, and OVCAR3 (Figure S1A). The cells were treated with CPI-613 and CD133/CD117 levels, and cell viability were measured 7 days following the treatment by flow cytometry. Initially, we conducted dose-response curves with CPI-613 with all cell lines. Based on our results, we selected 75 M as our treatment concentration, given that it was near the IC50 (half maximal inhibitory concentration) for all cell lines. Carboplatin/paclitaxel treatment was used as a positive control since it has been demonstrated to increase the frequency of CSCs [55]. Initially, we…