EGFR was immunoprecipitated from EGF treated or untreated HeLa cell lysates, and proteins were separated and immunoblotted (MADD can constitutively bind to TNFR1, and upon TNF binding to the TNFR, MADD facilitates quick recruitment of Grb2 to TNFR1 that leads to the activation of Ras and other downstream MAPK signaling molecules
EGFR was immunoprecipitated from EGF treated or untreated HeLa cell lysates, and proteins were separated and immunoblotted (MADD can constitutively bind to TNFR1, and upon TNF binding to the TNFR, MADD facilitates quick recruitment of Grb2 to TNFR1 that leads to the activation of Ras and other downstream MAPK signaling molecules. impact epidermal growth factor-induced MAPK activation thereby demonstrating the specific requirement of MADD for TNF receptor-mediated Imidaprilate MAPK activation. Re-expression of short hairpin RNA-resistant MADD in the absence of endogenous expression rescued the cells from TNF-induced apoptosis. The requirement for MADD was highly specific for TNF-induced activation of MAPK but not the related JNK and p38 kinases. Loss of MADD expression resulted in reduced Grb2 and Sos1/2 recruitment to the TNFR1 complex and decreased Ras and MEKK1/2 activation. These…