Cells were allowed to proliferate at 33C, 5% CO2 for 3 days, then switched to 37C, 5% CO2 for another 3 days for differentiation, with media changes every other day
Cells were allowed to proliferate at 33C, 5% CO2 for 3 days, then switched to 37C, 5% CO2 for another 3 days for differentiation, with media changes every other day. at the boundary between the lumen of the artificial microvessel and the collagen scaffold within the brain microvessel-on-a-chip. Video_3.avi (21M) GUID:?7E4EC0D7-419A-49FE-A6F8-0AEF99B8E3EE MOVIE S4: TY10 cells establish a functional barrier in the brain microvessel-on-a-chip. Time series of the fluorescence intensity presented as a heat map of antibody hmAb-AF568 diffusing from the lumen through the collagen as a function of time obtained at a flow of 1 1 l/min. Data was obtained in the presence of a monolayer of TY10 cells at the boundary between the lumen of the artificial microvessel and the collagen scaffold within the brain microvessel-on-a-chip. Video_4.avi (25M) GUID:?30C7DE07-BB88-4CE8-BC33-8074C5EC4E57…