Representative immunofluorescence images for every correct time point are shown, using the GFP sign revealing the localization from the VSV-G protein
Representative immunofluorescence images for every correct time point are shown, using the GFP sign revealing the localization from the VSV-G protein. We following investigated how GM130 KO cells behave within Apratastat a wound therapeutic assay, which may be used to measure the ability of cells to move directionally, polarize and migrate [22]. stage.(TIF) pone.0215215.s002.tif (847K) GUID:?A25E866D-02E1-4A36-A2F9-19B13AA8DF41 S3 Fig: GM130 isn't essential for centrosome structure maintenance. GM130 and Wild-type KO cells were stained with antibodies against centrin2 and Kendrin to visualize centrosome structure. Magnified pictures are proven in the containers. Size 10m.(TIF) pone.0215215.s003.tif (1.3M) GUID:?17D0A404-CF10-4141-A2BB-86F8A46812E6 S4 Fig: GM130 isn't essential for microtubule organization. (A) Wild-type and GM130 KO cells had been incubated on glaciers for 40 mins to depolymerize microtubules. Cells had been then used in room temperatures for three minutes…