Generating Osteoblasts from Murine Embryonic Stem Cell
by Emily Cooley

Murine embryonic stem cells were induced to differentiate into osteoblasts in the presence of vitamin D3, ?-glycerophosphate and ascorbic acid after 30 days in culture. This was confirmed through immunocytochemistry, von kossa staining and calcium assays. RNA collected during 10 specific time points during the 30 day differentiation were then used to investigate the activities of certain proteins during different times in the differentiation. ?-catenin and Wnt5a were both identified using Reverse Transcription PCR analysis.