Depletion of HAS2 in HCT116 and DLD1 cells, which express high levels of HAS2, critically increased sensitivity of radiation/oxaliplatin\mediated apoptotic cell death. the novel mechanisms behind the constitutive activation of HAS2 signaling in CRC, thereby highlighting its potential as a therapeutic target. values?Rabbit Polyclonal to ACSA CRC cells that express comparatively low levels of HAS2 (Figure?1E). Taken together, these results demonstrate that HAS2 levels correlate with the malignant phenotype of CRC. Open in a separate window Figure 1 Correlation of hyaluronic acid synthase 2 (HAS2) with the colorectal cancer (CRC) tissues and cell lines. Zaldaride maleate A, Immunohistochemistry of HAS2 expression in nonCneoplastic and CRC tissue samples (n?=?32) and scores for HAS2 staining. Scale bars: 50?m. B, Western blot analysis of HAS2 expression levels in 10 paired CRC and tumor\free tissue samples. (C) Western blot analysis and (D) immunofluorescence staining for Zaldaride maleate comparison Zaldaride maleate of HAS2 levels in CRC cell lines. E, Flow cytometric analysis of the apoptosis rate in CRC cell lines after therapeutic treatment (irradiation?=?10?Gy, Oxaliplatin?=?40?mol/L). Data are presented as mean??SD from one of three independent experiments with similar results. *P?P?P?P?P?P?